Human Anti-COL2A1 Antibody (CIIC1)


Product number #11310

Affinity purified human monoclonal anti- citrullinated collagen type II antibody clone CIIC1 in PBS, sterile filtered (0.2 µm)


10190 ACC4
Size and Price

100 µg / 0.1mL       200 €

1 mg / 1mL              1000 €

For bulk orders, please contact info@vacara.se to request a quote.

 Price is excluding shipping cost. The shipping cost will be added on.


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Anti-COL2A1 Antibody (CIIC1) binds to the triple helical C1 epitope on collagen type 2 (CII), but not when the epitope is double-citrullinated (Cit-Cit). CIIC1 can be used in studies of collagen citrullination together with mouse Anti-citrullinated COL2A1 Antibody (ACC4, Product number #10190). Binding specificity to triple helical C1 peptide epitopes was determined by bead-based flow immunoassay as median fluorescence intensity (MFI).

Description

Affinity purified chimeric human-mouse monoclonal anti collagen II (COL2A1) antibody clone CIIC1 in PBS. The CIIC1 antibody binds specifically to the C1 epitope on triple helical type 2 collagen. This clone has been used in applications including ELISA, immunohistochemistry (IHC) and in vivo functional studies such as collagen antibody induced arthritis1-3.

Target with alternative names: COL2A1, Collagen alpha-1(II) chain, CII, Col II. UniProt: P28481.

Immunogen: The antibody clone CIIC1 originates from B cells of collagen type 2 immunized DBA/1 mice1-3.

Epitope:  The CIIC1 antibody binds to the C1 epitope (position 358–363; GARGLT) on triple helical type 2 collagen 4,5.

Species reactivity: Mouse, rat, chicken, horse, baboon

Isotype:  Human IgG1, κ

Concentration: 1 mg/ml

Supplied in: PBS

Storage: Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Recommended dilution: It is recommended the user determines the optimal dilution for their application. The typical starting working dilution for IHC is 1:100 and 1:1000 for ELISA.

 

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

 

References

  1. Holmdahl, R., et al. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum. 1986. 29: 400–410. PMID: 2421741.
  2. Nandakumar KS, et al. Collagen type II-specific monoclonal antibody-induced arthritis in mice: description of the disease and the influence of age, sex, and genes. Am J Pathol. 2003 Nov;163(5):1827-37. PMID: 14578183.
  3. Nandakumar KS, et al. Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells. Arthritis Res Ther. 2004;6(6):R544-50. PMID: 15535832.
  4. Uysal H, et al. The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: structure to function analysis. Mol Immunol. 2008 Apr;45(8):2196-204. PMID: 18241923.
  5. Böiers U, et al. Collagen type II is recognized by a pathogenic antibody through germline encoded structures. Eur J Immunol. 2008 Oct;38(10):2784-95. PMID: 18825755.

Documents

Vacara_CIIC1.pdf