Mouse Anti-citrullinated COL2A1 Antibody (ACC4)
Product number #10190
Affinity purified mouse monoclonal anti- citrullinated collagen type II antibody clone ACC4 in PBS, sterile filtered (0.2 µm)
100 µg / 0.1mL 200 €
1 mg / 1mL 1000 €
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The mouse Anti-citrullinated COL2A1 Antibody clone ACC4 binds to the C1 epitope on alpha-chain of collagen II when the first arginine in the epitope (position 359–369; ARGLTGRPGDA) is citrullinated (Cit-Arg). The antibody does not bind to the C1 epitope when the second arginine is citrullinated (Arg-Cit), neither when both arginines are citrullinated (Cit-Cit). The antibody binds to the epitope in both triple helical and cyclic form. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI).
Description
Affinity purified mouse monoclonal anti collagen II (COL2A1) antibody clone ACC4 in PBS, sterile filtered (0,2 µm). The ACC4 antibody is an anti-citrullinated protein antibody (ACPA) that on COL2A1 binds specifically to citrullinated C1 epitope on alpha-chain. This antibody has been used in applications including immunohistochemistry (IHC) and in vivo functional studies such as collagen antibody induced arthritis and evoked pain-like behavior study1, 2.
Target with alternative names:
COL2A1,
Collagen alpha-1(II) chain, CII, Col II, citrullinated CII; citrullinated Col
II; citrullinated proteins. UniProt: P28481.
Immunogen: The antibody was generated by immunization of DBA/1 or (B10.Q × DBA/1) F1 mice with Protein-arginine deiminase type-4 (PAD4) -treated triple helical CII peptides containing T and B cell epitopes2.
Epitope: The ACC4 antibody is specific to citrullinated C1 epitope (position 359–369; ARGLTGRPGDA) on type 2 collagen alpha chain.
Species reactivity: Mouse, bovine and potentially human
Isotype: Mouse IgG1, κ (Other isotype available: ACC4-IgG2b)
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at
+4°C short term (1-2 weeks). Aliquot and store at -‑20°C long term. Avoid repeated
freeze/thaw cycles.
Recommended dilution: It is recommended the user determines the optimal dilution for their
application. The typical starting working dilution for IHC is 1:100.
For Research
Use Only. Not for use in diagnostic procedures.
Not for resale
without express authorization.
References
1. He, Y., Ge, C., Moreno-Giró, À. et al. A subset of antibodies targeting citrullinated proteins confers protection from rheumatoid arthritis. Nat Commun 14, 691 (2023). PMID: 36754962
2. Uysal, H. et al. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J. Exp. Med. 206, 449–462 (2009). PMID: 19204106
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