Mouse Anti-Col2A1 Antibody (M2139)
Product number #10110
Affinity purified mouse monoclonal anti-collagen II antibody clone M2139 in PBS, sterile filtered (0.2 µm)
100µg / 0.1mL 249 €
1 mg / 1mL 1,200 €
For bulk orders, please contact info@vacara.se to request a quote.
Price is excluding shipping cost. The shipping cost will be added on.
M2139 binds to the triple helical J1 epitope on collagen II. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI). Triple helical (Gly-Pro-Hyp)x11 is a synthetic control peptide with 11 repeats of the Gly-Pro-Hyp sequence and mimics the collagen II backbone structure.
Description
Affinity purified
mouse monoclonal anti collagen II (COL2A1) antibody clone M2139 in PBS, sterile
filtered (20 µm). The antibody recognizes the J1 epitope on triple helical
collagen II1. The J1 epitope on mouse collagen consist of residues
551–564 with sequence MPGERRGAAGIAGPK1,4. The antibody M2139 has
been used in applications including immunohistochemistry4, Western
blot2, immunoprecipitation3, and in vivo4
and in vitro5 functional assays.
Target with
alternative names: Collagen alpha-1(II)
chain, COL2A1, type II collagen, CII; UniProt: P28481.
Immunogen: Mouse collagen II J1 epitope (triple helical position 551-564) with
sequence MPGERRGAAGIAGPK1,4.
Species
reactivity: Mouse, rat, human,
bovine, chicken
Isotype: Mouse IgG2b, κ
Specificity: The antibody M2139 recognizes the conformational J1 epitope on the
triple-helical structure of the native CII molecule.
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2
weeks). Aliquot and store at 20°C long term. Avoid repeated freeze/thaw cycles.
Recommended
dilution: It is recommended the user determines the
optimal dilution for their application. The typical starting working dilutions
are: Functional assays (cell culture) 1:20–1:50, IHC 1:100.
For Research Use Only. Not for use in
diagnostic procedures.
Not for resale without express authorization.
References
1.
Mo JA, Holmdahl R. The B
cell response to autologous type II collagen: biased V gene repertoire with V
gene sharing and epitope shift. J Immunol. 1996 Sep 15;157(6):2440-8. PMID: 8805643.
2.
Tong D, Lönnblom
E, Yau ACY, Nandakumar KS, Liang B, Ge C, Viljanen J, Li L, Bãlan M, Klareskog
L, Chagin AS, Gjertsson I, Kihlberg J, Zhao M, Holmdahl R. A Shared Epitope of
Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and
Humans with Arthritis. Front Immunol. 2018 Apr 12;9:451. PMID: 29706949.
3.
Uysal H, Bockermann R,
Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H,
Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific
for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009
Feb 16;206(2):449-62. PMID: 19204106.
4.
Li Y, Tong D, Liang P,
Lönnblom E, Viljanen J, Xu B, Nandakumar KS, Holmdahl R. Cartilage-binding
antibodies initiate joint inflammation and promote chronic erosive arthritis.
Arthritis Res Ther. 2020 May 24;22(1):120. PMID: 32448385.
5.
Amirahmadi SF,
Whittingham S, Crombie DE, Nandakumar KS, Holmdahl R, Mackay IR, van Damme MP,
Rowley MJ. Arthritogenic anti-type II collagen antibodies are pathogenic for
cartilage-derived chondrocytes independent of inflammatory cells. Arthritis
Rheum. 2005 Jun;52(6):1897-906. PMID: 15934095.
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