Mutated Anti-Citrullinated Protein Antibody (E4NG-mutant)

Product number #10180

Suggested control antibody for E4NG (#10170)


This antibody is the mutated version of the E4NG antibody, with identical sequence, methods of production/purification and format to E4NG except two mutations in the paratope (W48M & S51A)1. This antibody has neglectable or no affinity to citrulline and does not have known cross-reactivity to other antigens1.

The E4NG-mutant antibody is recommended to be used as a control for E4NG antibody (#10170) in assays detecting citrulline-specificity of anti-citrullinated protein antibodies (ACPA) and is packed with product #10170 only and not sold separately. 


Mouse IgG2b, κ


100 µg/ml

Sizes available

50 µg/ 0.5 ml

100 µg/ 1.0 ml

Supplied in



Centrifuge briefly prior to opening the vial. Store at +4°C short term (1-2 weeks). Aliquot and store at ‑20°C long term. Avoid repeated freeze/thaw cycles.

Recommended dilution

It is recommended the user determines the optimal dilution with E4NG for their application.


For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.



1.      He, Y., Ge, C., Moreno-Giró, À. et al. A subset of antibodies targeting citrullinated proteins confers protection from rheumatoid arthritis. Nat Commun 14, 691 (2023).