Mouse Anti-citrullinated COL2A1 Antibody (ACC1)
Product number #10130
Affinity purified mouse monoclonal anti- citrullinated collagen type II antibody clone ACC1 in PBS, sterile filtered (0.2 µm)
100µg / 0.1mL 200 €
1 mg / 1mL 1000 €
For bulk orders, please contact info@vacara.se to request a quote.
Price is excluding shipping cost. The shipping cost will be added on.
The Mouse Anti-citrullinated COL2A1 Antibody clone ACC1 binds to the triple helical C1 epitope on collagen II when citrullinated. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI).
Description
Affinity purified mouse monoclonal
anti collagen II (COL2A1) antibody clone ACC1 in PBS, sterile filtered
(0.2 µm). The antibody binds to the citrullinated triple helical collagen
type II (CII) epitope (position 359-369; ARGLTGRPGDA)1, the C1
epitope. The ACC1 antibody recognizes the second citrulline at position 365 in
mouse CII1. The antibody binds to flexible triple-helical CII
determinants as well as many citrullinated CII peptides and was found to
cross-react with several non-citrullinated epitopes on native CII2. The
antibody has been used in applications including ELISA1,2, immunohistochemistry (IHC)1
and bead-based multiplex immunoassays2.
Target with alternative names:
COL2A1,
Collagen alpha-1(II) chain, CII, Col II, citrullinated CII; citrullinated Col
II; citrullinated proteins. UniProt: P28481.
Immunogen: Bovine Collagen type II 1
Species reactivity: Mouse, human
Isotype: Mouse IgG2a, κ
Specificity: ACC1 antibody binds the citrullinated C1 epitope. The ACC1 antibody
cross-reacts with triple-helical epitopes on CII, but not with other collagens
or the proteins tested in Ge et al2, except for a few citrullinated
peptides.2
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at
+4°C short term (1-2 weeks). Aliquot and store at -‑20°C long term. Avoid repeated
freeze/thaw cycles.
Recommended dilution: It is recommended the user determines the optimal dilution for their
application. The typical starting working dilutions are: IHC 1:100, ELISA 1:200
- 1:500.
For Research
Use Only. Not for use in diagnostic procedures.
Not for resale
without express authorization.
References
1. Uysal H, Bockermann R,
Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H,
Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific
for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009
Feb 16;206(2):449-62. PMID: 19204106.
2. Ge C, Tong D, Liang B,
Lönnblom E, Schneider N, Hagert C, Viljanen J, Ayoglu B, Stawikowska R, Nilsson
P, Fields GB, Skogh T, Kastbom A, Kihlberg J, Burkhardt H, Dobritzsch D,
Holmdahl R. Anti-citrullinated protein antibodies cause arthritis by
cross-reactivity to joint cartilage. JCI Insight. 2017 Jul 6;2(13):e93688.
PMID: 28679953.
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