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Native Cartilage Oligomeric Matrix Protein, recombinant human (rhCOMP)

Product number #10020 


Affinity purified recombinant human COMP in PBS, sterile filtered (0.2 µm)


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Product details

Cartilage Oligomeric Matrix Protein (COMP) Induced Arthritis in the C57BL/6 Mouse - The Methodology

Genetically modified mouse strains have most often been developed on the C57BL/6 genetic background, which has restricted their use for rheumatology research due to the lack of reliable model for rheumatoid arthritis (RA) for this strain. The C57BL/6 mice possess the MHC class II haplotype H‑2b, which has so far prohibited induction of any known model for rheumatoid arthritis.


Cartilage Oligomeric Matrix Protein (COMP) Induced Arthritis is a new model for rheumatoid arthritis in the C57BL/6 mouse. It is established by immunization by native COMP and leads to severe arthritis with characteristic T and B cell responses against defined COMP peptides. COMP induced arthritis provides disease model to study the immune priming, the effector and chronic phases of arthritis and can be used for efficient induction of arthritis in the C57BL/6J mice.


The key for successful arthritis experiments to solve your research questions is a thorough understanding of the model with its advantages and limitations.


Download This White Paper to Understand:

How to induce experimental arthritis in your C57BL/6 mouse

he expected severity and incidence of the disease

Differences between COMP models using IFA or CFA as adjuvant

Importance of the conformation of COMP for the model

The methodology to follow arthritis development ex vivo and in vitro

The benefits and limitations of COMP induced arthritis


Keywords: rheumatoid arthritis, cartilage oligomeric matrix protein, C57BL/6 mice

  Click here to download the White Paper

FAQs

Q1: What does 'native recombinant' COMP mean?

  • Native recombinant COMP is produced in expression systems that preserve its natural pentameric structure and post-translational modifications, typically using mammalian cells such as HEK293 or CHO

Q2: Is our recombinant COMP present in native state?

  • Yes. Our recombinant COMP is in native state, validated using the 16B5 antibody, which specifically binds the coiled-coil domain and pentameric structure of COMP.


Q3: Can native recombinant COMP be used as an ELISA standard?

  • Yes. High-purity native recombinant COMP is widely used as a standard for serum COMP quantification assays in osteoarthritis and rheumatoid arthritis biomarker studies.


Q4: Is it important to have native COMP to induce arthritis?

  • Yes. Using native recombinant COMP is critical for reliably inducing COMP-induced arthritis. For more details refer to Zhao et al. (2021).

Q5: What types of research benefit from native COMP?

  • Autoantigen and epitope mapping
  • B-cell and T-cell immune response studies
  • COMP-induced arthritis model development
  • Therapeutic testing (B-cell, complement, cytokine inhibitors)
  • Biomarker validation for cartilage turnover

Q6: Was the biological activity tested on our COMP?

  • Biological activity assays was not performed. Only the size and status of the recombinant COMP were evaluated using ELISA and SDS-PAGE.

Q7: What kind of mouse strains can be used for COMP-induced arthritis?

  • Several mouse strains have been used in COMP-induced arthritis (COMPIA), supported by published studies:

-    C57BL/6 (B6N) (Zhao Y et al., Frontiers in Immunology (2021) -Recommended

-    DBA/1 (Carlsen S. et al., Arthritis & Rheumatology (2008)

-    B10.Q ( Carlsen S. et al., Arthritis & Rheumatology (2008)