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Mouse Anti-Col2A1 Antibody (M2139)

Mouse Anti-Col2A1 Antibody (M2139)

Product number #10110


Affinity purified mouse monoclonal anti-collagen II antibody clone M2139 in PBS, sterile filtered (0.2 µm)


Size and Prices

100 µg / 0.1 mL249 €
1 mg / 1 mL1,200 €


For bulk orders, please contact info@vacara.se to request a quote.

Price is excluding shipping cost. The shipping cost will be added on.

Product details

M2139 binds to the triple helical J1 epitope on collagen II. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI). Triple helical (Gly-Pro-Hyp)x11 is a synthetic control peptide with 11 repeats of the Gly-Pro-Hyp sequence and mimics the collagen II backbone structure.


Description

Affinity purified mouse monoclonal anti collagen II (COL2A1) antibody clone M2139 in PBS, sterile filtered (0.2 µm). The antibody recognizes the J1 epitope on triple helical collagen II1. The J1 epitope on mouse collagen consists of residues 551–564 with sequence MPGERRGAAGIAGPK1,4. The antibody M2139 has been used in applications including immunohistochemistry4, Western blot2, immuno­precipitation3, and in vivo4 and in vitro5 functional assays.


Target with alternative names

Collagen alpha-1(II) chain, COL2A1, type II collagen, CII; UniProt: P28481.


Immunogen

Mouse collagen II J1 epitope (triple helical position 551-564) with sequence MPGERRGAAGIAGPK1,4.


Species reactivity 

Mouse, rat, human, bovine, chicken


Isotype 

Mouse IgG2b, κ


Specificity

The antibody M2139 recognizes the conformational J1 epitope on the triple-helical structure of the native CII molecule.


Concentration

1 mg/ml


Size

100µg / 0.1 ml

1 mg / 1 ml


Supplied in

PBS


Storage

Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at ‑20°C long term. Avoid repeated freeze/thaw cycles.


Recommended dilution

It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: Functional assays (cell culture) 1:20-1:50, IHC 1:100.


For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

References

  1. Mo JA, Holmdahl R. The B cell response to autologous type II collagen: biased V gene repertoire with V gene sharing and epitope shift. J Immunol. 1996 Sep 15;157(6):2440-8. PMID: 8805643.
  2. Tong D, Lönnblom E, Yau ACY, Nandakumar KS, Liang B, Ge C, Viljanen J, Li L, Bãlan M, Klareskog L, Chagin AS, Gjertsson I, Kihlberg J, Zhao M, Holmdahl R. A Shared Epitope of Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and Humans with Arthritis. Front Immunol. 2018 Apr 12;9:451. PMID: 29706949.
  3. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62. PMID: 19204106.
  4. Li Y, Tong D, Liang P, Lönnblom E, Viljanen J, Xu B, Nandakumar KS, Holmdahl R. Cartilage-binding antibodies initiate joint inflammation and promote chronic erosive arthritis. Arthritis Res Ther. 2020 May 24;22(1):120. PMID: 32448385.
  5. Amirahmadi SF, Whittingham S, Crombie DE, Nandakumar KS, Holmdahl R, Mackay IR, van Damme MP, Rowley MJ. Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells. Arthritis Rheum. 2005 Jun;52(6):1897-906. PMID: 15934095.

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FAQs

Q1: Why choose M2139 over polyclonal anti-collagen II antibodies?

  • M2139 is a monoclonal antibody that binds a single, well-defined epitope (J1: residues 551–564) on the triple-helical region of native collagen type II. This ensures minimal cross-reactivity with other collagen types (e.g., I, III, XI), unlike polyclonals which may bind multiple epitopes across different collagens. Monoclonal antibody M2139 works  consistently from batch-to-batch and is conformation sensitive: M2139 recognizes the native triple-helical form, making it ideal for detecting intact collagen II in tissue sections or functional assays.

Q2: Which secondary antibody should I use with M2139?

  • M2139 is a mouse IgG2b isotype and an anti-mouse IgG2b or anti-mouse IgG  work as a secondary antibody. Choose the conjugate for your application. For  example, for immunohistochemistry (IHC) an anti-mouse HRP- or AP conjugate is appropriate. For immunofluorescence (IF) and flow cytometry (FCM) an anti-mouse IgG2b fluorophore-conjugate chosen based on your setup is the choice. 

Q3: Can M2139 induce arthritis in experimental models?

  • Yes. M2139 has been used to induce collagen antibody-induced arthritis (CAIA) in mice when co-administered with lipopolysaccharide (LPS), making it valuable for autoimmune arthritis research. M2139 is a component of Cab3 and Cab4 cocktails of cartilage-binding antibodies that initiate joint inflammation in preclinical models  of arthritis (PMID: 32448385)

Q4: How does M2139 compare to other monoclonals like CIIC1 or 5B2?

  • M2139’s defined epitope, arthritis-inducing capability, and low cross-reactivity make it a gold standard for native collagen II detection and autoimmune arthritis models. M2139 binds to the J1 epitope regardless of citrullination , whereas binding of anti-collagen antibody CIIC1 may be abolished by double citrullination. M2139 binds to the well-defined J1 epitope on COL2A1, whereas the 5B2 binds the N-terminal telopeptide (PMID: 7687475). 

Q5: Do you provide tagged versions of this antibody?

  • Yes. For example biotinylated M2139 is available upon request. Please contact us at info@vacara.se with your specific needs to know more!