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Cab3 and Cab4 cocktails

Cartilage Antibody Cocktail (Cab3)

Product number #10030 


Affinity purified mouse anti-mouse cartilage monoclonal antibodies in PBS, sterile filtered (0.2 µm)


Size and Prices

Please contact info@vacara.se to request a quote.

Cartilage Antibody Cocktail (Cab4)

Product number #10040 


Affinity purified mouse anti-mouse cartilage monoclonal antibodies in PBS, sterile filtered (0.2 µm)


Size and Prices

Please contact info@vacara.se to request a quote.


Product details

Immunohistochemical staining to investigate antibody binding to cartilage in vivo


Cab3 and Cab4 cocktails are pools of three and four arthritogenic antibodies, respectively, with antibodies known to bind cartilage and initiate arthritis in several mouse strains, including the commonly used C57BL/6 strain. Biotinylated versions of the antibodies may be administered in vivo, and avidin-conjugated reagents can then be used to visualize and quantify the in vivo -bound antibodies in the tissue processed ex vivo for confocal microscopy. The methodology to detect the cartilage-bound antibodies may be adapted to investigate the timing and fate of the arthritogenic antibodies and may be supplemented with immunohistochemical method to detect complement deposited on the cartilage. This information may be utilized to investigate the mechanisms of arthritis development in preclinical models.

Download This White Paper to Understand:

  • How to collect and prepare the limbs for immunohistochemical staining
  • The steps to perform immunohistochemical staining of mouse arthritic joints for fluorescence microscopy to visualize bound antibodies or complement C3 on the cartilage
  • What are the Do’s and Don’ts in immunohistochemical staining methodology

  Click here to download the White Paper

FAQs

Q1: What antibodies are included in the Cab 3 and Cab 4 cocktails?

  • The Cab3 antibody cocktail contains equal parts of M2139, L10D9, and 15A11, while the Cab4 cocktail includes M2139, L10D9, 15A11, and ACC1. Each cocktail is mixed in equal amounts prior to use to ensure consistent performance. Full details on isotype, epitope specificity, and validation data are available in our product white paper.


Q2: What mouse strains are compatible with Cab3 and Cab4?

  • Cab3 and Cab4 are validated for use in C57BL/6 and several other commonly used inbred mouse strains. Their strong cartilage-binding affinity and cross-reactivity make them suitable for a broad range of preclinical rheumatoid arthritis and cartilage-targeting studies.


Q3: What can the Cab3 and Cab 4 be used for?

  • Both antibody cocktails are compatible with standard immunohistochemistry (IHC) and ELISA protocols. They are validated for in vitro detection of cartilage antigens, and can be incorporated into routine fixation and staining workflows. For best results, refer to the recommended protocol provided in the datasheet or reach out to our scientific support team.


Q4: Are Cab3 and Cab4 available in biotinylated or labeled forms?

  • Vacara offers custom formats, including biotinylated or fluorescently labeled versions upon request. Contact us to discuss custom antibody formulations for your specific research needs.